Péppin' Lê Pew

Gather materials

• 3 × ⅝” needles
• 1 × 1 ½” needle
• sterile alcohol wipes
• 1 × 3 ml syringe
• 1 × sterile 0.22μm filter
• X peptide vials containing X mg each
• Target concentration: X mg/ml
• Total BAC water: X ml, plus some extra for purging and flushing
• 1 × sterile empty cartridge/vial
• 1 × pen needle

1. Sanitize everything with 70% IPA
2. Wipe down the cartridge and vial tops with sterile wipes
3. Draw up X ml + 0.3 ml = X ml of BAC, or more
4. Attach filter and ⅝” needle to BAC. Purge all air out of filter and needle
5. Insert the needle + filter into the cart. Leave this needle + filter in the cart until the entire process is complete
6. Remove the syringe (with all remaining BAC) from the filter, leaving the filter attached to the cart
7. Attach a new 1 ½” needle
8. Purge so there is X ml left in the syringe. Note: we’re reserving 0.5 ml for the final filter flush
9. Split that X ml evenly between the peptide vials. That’s approximately X ml in each vial. The syringe should now be empty
10. Cap the needle. Swirl the vials gently and wait 30 minutes, or at least 10 minutes more than you think you should (this allows microsocopic particles to dissolve)
11. Uncap the needle and draw up the X ml of dissolved peptide solution from all the vials
12. Remove the needle and discard it
13. Get your ⅝” vent needle ready
14. Attach the syringe to the filter/cart
15. Gently press about 0.5 ml into the cart to create slight positive pressure. Do not over-pressurize or you risk blowing the plug out the bottom of the cartridge. The amount is not critical.
16. Insert a ⅝” needle as a vent into the cart. Doing it in this order minimizes contamination risk; internal pressure keeps airflow one direction in the vent needle
17. Finish injecting the rest of the peptide solution into the cart. Venting prevents excess pressure buildup
18. We are still 0.5 ml short. Using a new ⅝” needle (never reuse a needle in the BAC vial), draw up 0.5 ml of BAC water. Disconnect that needle and discard it, reattach the syringe to the filter, and push the last 0.5 ml through to flush the filter
19. Remove the vent needle and the filter needle
20. Attach a pen needle and purge all air from the cart
21. Detach the pen needle and discard. You're ready to go!